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News Archive |
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UK DEFRA LINK Food
Safety Programme
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RBR is currently part of a joint
UK DEFRA LINK Food Safety Programme /FSA funded project to
assess the Fate of Fusarium Mycotoxins in the Cereal Food
Chain.
One purpose of the project is to contribute data addressing the fate of
Fusarium mycotoxins during commercial food processing and assist with an
appraisal of legislative limits. The information generated from this project
should help food companies to comply with the new regulations and so help
them to best protect the consumer.
As part of the project a brief evaluation of RIDA®QUICK
DON, a lateral flow device for screening cereals for deoxynivalenol at
the EU proposed legislative limits was performed. 12 participants took
part in the study and each were provided with 9 blind samples of cereals
including wheat, maize, barley and oats for testing using RIDA®QUICK
DON at 500ppb. One positive sample, Maize 73 was provided to confirm that
there was no problem in operating the test.
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With RIDA®QUICK
DON the presence of a test line following analysis represents
a positive result above the 500ppb cut off level. No line
indicates a negative result below the cut off level of the
test. The screening level of the test can also be adjusted
by changing the sample dilution prior analysis.
Out of 108 analyses, 107 agreed with the HPLC reported result for each
sample and except in one instance, the presence of DON at 500 ppb or above
was correctly identified.
A positive for maize at 100 ppb was obtained by one participant and confirmed
by repeating.
In conclusion the RIDA®QUICK DON lateral
flow device was found to be suitable for screening a range of cereals above
or below a 500ppb level and is recommended for use by the cereal industry. |
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| Sample
No |
DON
Cont.
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Lab
1
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Lab
2
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Lab
3
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Lab
4
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Lab
5
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Lab
6
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Lab
7
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Lab
8
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Lab
9
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Lab
10
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Lab
11
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Lab
12
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| Maize 680 |
750ppb
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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| Maize 730 |
<10ppb
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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| Maize 731 |
500ppb
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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| Oats 833 |
500ppb
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>500
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<500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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| Barley 1 |
<10ppb
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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| Wheat 786 |
<10ppb
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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| Wheat 1002 |
>1000ppb
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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| Maize 327 |
100ppb
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<500
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<500
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<500
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<500
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<500
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>500
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<500
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<500
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<500
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<500
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<500
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<500
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| Oats 456 |
<10ppb
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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<500
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| Maize 73 |
>500ppb
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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>500
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| © 2002 R-Biopharm Rhone Ltd |
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