News Archive

 April 2007
Take the Toxin Challenge!

In order to help companies keep their products in line with current EU legislation, R-Biopharm Rhône Ltd have a range of immunoaffinity columns available for the analysis of mycotoxins and vitamin B12. The columns are very sensitive, detecting below the limits specified in the regulations and their ease of use and flexibility make them an ideal addition to any laboratory.

We would like to take this opportunity to offer customers the chance to try another immunoaffinity column from the RBR range for sample clean-up prior to HPLC analysis. Perhaps now that your laboratory have established methods for aflatoxins and ochratoxin A, is it time to look at the other mycotoxins such as T2 and HT-2, deoxynivalenol, zearalenone, and fumonisin? Alternatively maybe you are just starting to analyse for aflatoxins or vitamin B12 for the first time using HPLC and would like to see how AFLAPREP® or EASI-EXTRACT® Vitamin B12 works?

During the month of April R-Biopharm Rhône Ltd are offering a free sample pack containing 5 immunoaffinity columns complete with QC certificate and instructions for use to encourage labs to try other types of analysis. If you would like to take advantage of this great offer, simply complete the fax back form overleaf and tick the box of the immunoaffinity column you would like to evaluate and we will arrange for the columns to be delivered.

In order to help us with our ongoing product development, we would be very interested to know your thoughts on the products, your likes and dislikes and if there are any ways in which we could improve them. By completing the evaluation form (included in the demo pack) and returning it to us, we can use this knowledge to continue to provide you with the best products on the market.

Choose from the following options:

Column Test Kit

Product Code

No. Of Tests
AFLAPREP®
P07
5
OCHRAPREP®
P14
5
AFLAPREP® M
P04
5
FUMONIPREP®
P31
5
DONPREP®
P50
5
EASI-EXTRACTION® AFLATOXIN

RP71

5
EASI-EXTRACTION® ZEARALENONE
RP91
5
EASI-EXTRACTION® T-2 & HT-2
P43
5
EASI-EXTRACTION® VITAMIN B12
P80
5

For further information please contact RBR by either fax or email if you have any questions regarding this offer.

Demo Pack Request Faxback Form   Download evaluation file
Update on Mycotoxin Regulations

Mycotoxins have been in the news on a number of occasions recently following the discovery of increased levels in corn in the US and the introduction of 3 new EU regulations coming into force this month.

In the US, the Indiana Statistics Service have reported the highest levels of mycotoxins in corn for 10 years. This analysis was carried out by the Indiana Animal Disease Diagnostic Laboratory who found deoxynivalenol levels ranging from 0.5-8ppm in samples from six counties and fumonisin B1 levels ranging from 0.7 to 174ppm.
These levels are of particular concern to pig farmers, as pigs are more sensitive than other animals and suffer severe effects including vomiting and reduced feed consumption.

The new EU regulations, which came into force on 1st March 2007, are part of a legislative drive to increase food safety with Regulation (EC) 1881/2006 setting maximum levels for specific contaminants in foodstuffs, which includes mycotoxins.

In preparation for these recommendations, a new method has been developed for the analysis of ochratoxin A in liquorice using Ochraprep immunoaffinity columns prior to analysis by HPLC. The method uses 1% sodium bicarbonate /deionised water to extract the toxin and then the column is washed with 10% tween 20 /PBS to remove pigments prior to elution. The toxin is then eluted from the column using acidified methanol and is injected onto the HPLC. The chromatograms produced using this method were found to be extremely clean considering the complexity and the colour of this sample matrix.Recoveries obtained were in excess of 75% confirming that the method is suitable to meet the proposed legislative limits.

In addition to the new method for liquorice, work is currently being carried out to produce application notes for other complex commodities using different test kits in our range. Method development is currently underway for the analysis of aflatoxins in cocoa using AFLACARD®, T2 and HT-2 in oats using EASI-EXTRACT® T2 and HT-2 and for Vitamin B12 using EASI-EXTRACT® Vitamin B12 columns for a variety of dairy products. So far the results with these methods look promising and the completed application notes should be available shortly.


For further information please contact RBR.